
Cambridge scientists have developed a new method for growing and transplanting artificial bile ducts that could in future be used to help treat liver disease in children, reducing the need for liver transplantation.听
Cambridge scientists have developed a new method for growing and transplanting artificial bile ducts that could in future be used to help treat liver disease in children, reducing the need for liver transplantation.听
Our work has the potential to transform the treatment of bile duct disorders
Ludovic Vallier
In research published in the journal Nature Medicine, the researchers grew 3D cellular structure which, once transplanted into mice, developed into normal, functioning bile ducts.
Bile ducts are long, tube-like structures that carry bile, which is secreted by the liver and is essential for helping us digest food. If the ducts do not work correctly, for example in the childhood disease biliary atresia, this can lead to damaging build of bile in the liver.
探花直播study suggests that it will be feasible to generate and transplant artificial human bile ducts using a combination of cell transplantation and tissue engineering technology. This approach provides hope for the future treatment of diseases of the bile duct; at present, the only option is a liver transplant.
探花直播 探花直播 of Cambridge research team, led by Professor Ludovic Vallier and Dr Fotios Sampaziotis from the Wellcome-MRC Cambridge Stem Cell Institute and Dr Kourosh Saeb-Parsy from the Department of Surgery, extracted healthy cells (cholangiocytes) from bile ducts and grew these into functioning 3D duct structures known as biliary organoids.听 When transplanted into mice, the biliary organoids assembled into intricate tubular structures, resembling bile ducts.
探花直播researchers, in collaboration with Mr Alex Justin and Dr Athina Markaki from the Department of Engineering, then investigated whether the biliary organoids could be grown on a 鈥榖iodegradable collagen scaffold鈥, which could be shaped into a tube and used to repair damaged bile ducts in the body.听 After four weeks, the cells had fully covered the miniature scaffolding resulting in artificial tubes which exhibited key features of a normal, functioning bile duct.听 These artificial ducts were then used to replace damaged bile ducts in mice.听 探花直播artificial duct transplants were successful, with the animals surviving without further complications.听
鈥淥ur work has the potential to transform the treatment of bile duct disorders,鈥 explains Professor Vallier. 鈥淎t the moment, our only option is liver transplantation, so we are limited by the availability of healthy organs for transplantation. In future, we believe it will be possible to generate large quantities of bioengineered tissue that could replace diseased bile ducts and provide a powerful new therapeutic option without this reliance on organ transplants.鈥
鈥淭his demonstrates the power of tissue engineering and regenerative medicine,鈥 adds Dr Sampaziotis. 鈥淭hese artificial bile ducts will not only be useful for transplanting, but could also be used to model other diseases of the bile duct and potentially develop and test new drug treatments.鈥
Professor Vallier is part of the Department of Surgery at the 探花直播 of Cambridge and his team are jointly based at the Wellcome Trust-MRC Cambridge Stem Cell Institute and the Wellcome Trust Sanger Institute.听
探花直播work was supported by the Medical Research Council, Sparks children鈥檚 medical research charity and the European Research Council.
Reference
Sampaziotis, F et al. Nature Medicine; 3 July 2017; DOI: 10.1038/nm.4360
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